Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering.
نویسندگان
چکیده
Enzymes are widely used for the synthesis of pharmaceuticals, agrochemicals, and food additives because they can catalyze enantioselective transformations. Understanding the molecular basis of enzyme–substrate interactions that contribute to enantioselectivity is important for constructing selective enzymes by protein engineering. Up to now, emphasis has been on reactions such as lipaseor esterase-based kinetic resolutions, as well as lyase-, aminotransferaseand ketoreductase-mediated conversions. An emerging group of enzymes that is explored for enantioselectivity is dehalogenases. Haloalkane dehalogenases can convert a broad range of halogenated aliphatic substrates to their corresponding alcohols by an SN2 mechanism (Scheme 1), [5]
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ورودعنوان ژورنال:
- Angewandte Chemie
دوره 49 35 شماره
صفحات -
تاریخ انتشار 2010